LOCUS       sequence-71985-e        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  TITLE     Series-12:  S cerevisiae Expression Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Gal1/10 His6-MBP TEV Trp S. cerevisiae express...
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..64
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     636..657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaacaacaac aacaataaca ataacaacaa cctcgggatc gaggaaaacc tgtacttcca
       61 atccaatatt ggaagtggat aactctcgag gatcgtaatg actagattat ataatcagaa
      121 atagcctttg gtgatcactt ccattttagt cagcataata gaaagtttaa ttacgcgctt
      181 ttttacattc cgacaaagat ctgcccgaga aaattcttcg agaagacaac aacaacaaaa
      241 aaaataaagc ctgggcgaaa aacatcgtgc gtttacataa tatcttgaaa agcaaacaga
      301 tatataaacc gctattgaaa ttaccttttt aagtattcgc ctagaagcct tgtttcaagt
      361 agcatcaaca acaggtgtta ggttaaactg ctttgacttt ttgttcttta acgaatatca
      421 ttaaaaaaaa aggacatcta taaagaacgc gtaattcctg cagcccggct ttccccgtca
      481 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
      541 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
      601 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
      661 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
      721 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaattatt
      781 aacgtttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      841 cgcatagatc ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat
      901 ttcttagcat ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc
      961 acacctccgc ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt
     1021 tctggcgtca gtccaccagc taacataaaa tgtaagcttt cggggctctc ttgccttcca
     1081 acccagtcag aaatcgagtt ccaatccaaa agtt
//
