LOCUS       sequence-7199-ex        4866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP, Burge CB, 
            Bartel DP
  TITLE     The widespread impact of mammalian MicroRNAs on mRNA repression and 
            evolution.
  JOURNAL   Science. 2005 Dec 16. 310(5755):1817-21.
  PUBMED    16308420
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2005 Dec 16. 310(5755):1817-21."
COMMENT     Sequence Label: pAG71 BDNF 3'UTR mut
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    29..150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(40..59)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     94..113
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        283..387
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(388..456,457..1248)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(606..625)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1419..2007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1908..1927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2136..2493
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2136..2327
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2159..2179)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2344..2479
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2563..2695
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2678..2697
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        2740..2758
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2768..3703
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 ctagagcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
       61 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
      121 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
      181 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
      241 aaaatcgata aggatccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      601 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
      661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
      961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     1081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     1141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1561 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1681 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1741 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1801 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1861 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1921 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1981 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2041 gccttttgct ggccttttgc tcacatggct cgacagatct gcgcagcacc atggcctgaa
     2101 ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg
     2161 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2221 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     2281 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     2341 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     2401 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     2461 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg attcttctga cacaacagtc
     2521 tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca
     2581 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt
     2641 ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc
     2701 cactcccagt tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct
     2761 agccaccatg acttcgaaag tttatgatcc agaacaaagg aaacggatga taactggtcc
     2821 gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta attattatga
     2881 ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg cctcttctta
     2941 tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta taccagacct
     3001 tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac ttgatcatta
     3061 caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca tttttgtcgg
     3121 ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag ataagatcaa
     3181 agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg aatggcctga
     3241 tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg ttttggagaa
     3301 taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag aaccagaaga
     3361 atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc caacattatc
     3421 atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac aaattgttag
     3481 gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta ttgaatcgga
     3541 cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta atactgaatt
     3601 tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa tgggaaaata
     3661 tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg agctccagtc
     3721 atttgcgcac aacttaaaaa gtctgcatta gaatgcttga taatgttgtg gtttgttgcc
     3781 gttgccaaga actgaaaaca taaaaagtta aaaaaaataa taaattgcat gctgctttaa
     3841 ttgtgaattg ataataaact gtcctctttc agaaaacaga aaaaaaacac acacacacac
     3901 aacaaaaatt tgaaccaaaa cattccgttt acattttaga cagtaagtat cttcgttctt
     3961 gttagtacta tatctgtttt actgctttta acttctgata gcgttggaat taaaacaatg
     4021 tcaaggtgct gttgtcattg ctttactggc ttaggggatg ggggatgggg ggtatatttt
     4081 tgtttgtttt gtgttttttt ttcgtttgtt tgttttgttt tttagttccc acagggagta
     4141 gagatgggga aagaattcct acaatatata ttctggctga taaaagatac atttgtatgt
     4201 tgtgaagatg tttgcaatat cgatcagatg actagaaagt gaataaaaat taaggcaact
     4261 gaacaaaaaa atgctcacac tccacatccc gtgatgcacc tcccaggccc cgctcattct
     4321 ttgggcgttg gtcagagtaa gctgcttttg acggaaggac ctatgtttgc tcagaacaca
     4381 ttctttcccc ccctccccct ctggtctcct ctttgttttg ttttaaggaa gaaaaatcag
     4441 ttgcgcgttc tgaaatattt taccactgct gtgaacaagt gaacacattg tgtcacatca
     4501 tgacactcgt ataagcatgg agaacagtga ttttttttta gaacagaaaa caacaaaaaa
     4561 taaccccaaa atgaagatta ttttttatga ggagtgaaca tttgggtaaa tcatggctaa
     4621 gcttaaaaaa aactcatggt gaggcttaac aatgtcttgt aagcaaaagg tagagccctg
     4681 tatcaaccca gaaacaccta gatcagaaca ggaatccaca ttgccagtga catgagactg
     4741 aacagccaaa tggaggctat gtggagttgg cattgcattt accggcagtg cgggaggaat
     4801 ttctgagtgg ccatcccaag gtctaggtgg aggtggggca tggtatttga gagaatgcaa
     4861 aacgat
//
