LOCUS       sequence-72009-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-YC3.60
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      72..81
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct tgccaccatg gttccagagc ccggccctgt caacagtagc accccagcct
      121 ggggccccgg gccaccgcca gccccagggg gcagcggctg ggtggccgct gcgctgtgcg
      181 tggtcatcgt gctgacggca gccgccaatt cgctgctgat cgcgctcatc tgcacgcagc
      241 ccgcgctgcg caacacgtct aacttcttcc tggtgtcgct cttcacgtcg gacctgatgg
      301 tgggattggt ggtgatgccc ccagccatgc tgaacgcgct gtatgggcgc tgggtactag
      361 ctcgcggcct ctgtctgctt tggaccgcct tcgacgtgat gtgctgcagc gcctccattc
      421 tcaacctctg cctcatcagc ctggaccgct acctgctcat cctctcgccg ctgcgctaca
      481 agctgcgcat gacagccccg cgtgccctgg cgctcatcct gggtgcctgg agcctcgccg
      541 cgctggcctc cttcctgccc ctcttgctgg gctggcatga actgggcaaa gctcgaacat
      601 ctgccccggg ccagtgccgc ctattggcca gcctgcctta tgtcctcgtg gcgtccggcg
      661 tcaccttttt cctgccttcg ggtgccatct gcttcaccta ctgcaggatc ttgctggctg
      721 cccgcaagca ggcggtgcaa gtggcctcgc tcaccacggg cacggctacg gctggccagg
      781 ccttggaaac cttgcaggtg ccca
//
