LOCUS       sequence-7201-ex        4111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP, Burge CB, 
            Bartel DP
  TITLE     The widespread impact of mammalian MicroRNAs on mRNA repression and 
            evolution.
  JOURNAL   Science. 2005 Dec 16. 310(5755):1817-21.
  PUBMED    16308420
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2005 Dec 16. 310(5755):1817-21."
COMMENT     Sequence Label: pAG79 3'UTR mut
FEATURES             Location/Qualifiers
     source          1..4111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    426..547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(437..456)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     491..510
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        680..784
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(785..853,854..1645)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1003..1022)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1816..2404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2305..2324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2533..2890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2533..2724
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2556..2576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2741..2876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2960..3092
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3075..3094
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        3137..3155
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3165..4100
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 cgtacaagcc attgaataag cctcttcctt ttttttgctc aaagaatgca catccttgtg
       61 gattcccctg cattgtttgt tttatataac atttgatatt tgttgtagct tgtatatgaa
      121 cataattttc tttagaggta gtcactgttc tctccagtat gacccaggtt tcttgactct
      181 gagtaatgca ccttctataa ctatctaaat ttctattgaa gctttttgga ttatgagtat
      241 gctgactttt cacgattggc tggtgcatgt ttagacttaa atgtcatatc cttcatgtct
      301 caaagccaaa atagtaacat ctcatctcag aacagagctg tgaccacatg ccaatatatg
      361 tgtcacaaag tctacatatg ttagaatgct tggaagtcta gagcggccgc ttcgagcaga
      421 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      481 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      541 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      601 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccaggtgg
      661 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      721 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      781 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      841 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      901 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      961 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1021 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1081 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1141 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1201 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1261 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1321 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1381 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1441 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1501 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1561 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1621 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1681 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1741 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1801 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1861 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1921 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1981 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2041 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2101 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2161 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2221 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2281 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2341 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2401 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2461 catggctcga cagatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     2521 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     2581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2641 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2701 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     2761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2821 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2881 cttttgcaaa aagcttgatt cttctgacac aacagtctcg aacttaagct gcagaagttg
     2941 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
     3001 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
     3061 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
     3121 ttaaggctag agtacttaat acgactcact ataggctagc caccatgact tcgaaagttt
     3181 atgatccaga acaaaggaaa cggatgataa ctggtccgca gtggtgggcc agatgtaaac
     3241 aaatgaatgt tcttgattca tttattaatt attatgattc agaaaaacat gcagaaaatg
     3301 ctgttatttt tttacatggt aacgcggcct cttcttattt atggcgacat gttgtgccac
     3361 atattgagcc agtagcgcgg tgtattatac cagaccttat tggtatgggc aaatcaggca
     3421 aatctggtaa tggttcttat aggttacttg atcattacaa atatcttact gcatggtttg
     3481 aacttcttaa tttaccaaag aagatcattt ttgtcggcca tgattggggt gcttgtttgg
     3541 catttcatta tagctatgag catcaagata agatcaaagc aatagttcac gctgaaagtg
     3601 tagtagatgt gattgaatca tgggatgaat ggcctgatat tgaagaagat attgcgttga
     3661 tcaaatctga agaaggagaa aaaatggttt tggagaataa cttcttcgtg gaaaccatgt
     3721 tgccatcaaa aatcatgaga aagttagaac cagaagaatt tgcagcatat cttgaaccat
     3781 tcaaagagaa aggtgaagtt cgtcgtccaa cattatcatg gcctcgtgaa atcccgttag
     3841 taaaaggtgg taaacctgac gttgtacaaa ttgttaggaa ttataatgct tatctacgtg
     3901 caagtgatga tttaccaaaa atgtttattg aatcggaccc aggattcttt tccaatgcta
     3961 ttgttgaagg tgccaagaag tttcctaata ctgaatttgt caaagtaaaa ggtcttcatt
     4021 tttcgcaaga agatgcacct gatgaaatgg gaaaatatat caaatcgttc gttgagcgag
     4081 ttctcaaaaa tgaacaataa ttctaggagc t
//
