LOCUS       sequence-72010-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      76..85
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttggt
       61 accgagctcg gatccgccac catggttcca gagcccggcc ctgtcaacag tagcacccca
      121 gcctggggcc ccgggccacc gccagcccca gggggcagcg gctgggtggc cgctgcgctg
      181 tgcgtggtca tcgtgctgac ggcagccgcc aattcgctgc tgatcgcgct catctgcacg
      241 cagcccgcgc tgcgcaacac gtctaacttc ttcctggtgt cgctcttcac gtcggacctg
      301 atggtgggat tggtggtgat gcccccagcc atgctgaacg cgctgtatgg gcgctgggta
      361 ctagctcgcg gcctctgtct gctttggacc gccttcgacg tgatgtgctg cagcgcctcc
      421 attctcaacc tctgcctcat cagcctggac cgctacctgc tcatcctctc gccgctgcgc
      481 tacaagctgc gcatgacagc cccgcgtgcc ctggcgctca tcctgggtgc ctggagcctc
      541 gccgcgctgg cctccttcct gcccctcttg ctgggctggc atgaactggg caaagctcga
      601 acatctgccc cgggccagtg ccgcctattg gccagcctgc cttatgtcct cgtggcgtcc
      661 ggcgtcacct ttttcctgcc ttcgggtgcc atctgcttca cctactgcag gatcttgctg
      721 gctgcccgca agcaggcggt gcaagtggcc tcgctcacca cgggcacggc tacggctggc
      781 caggccttgg aa
//
