LOCUS       sequence-72013-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV TALE synthesis backbone with HD half repeat; fused to nuclear 
            localization sequence linker and VP64 transcriptional activator 
            effector domain. Synapsin promoter for neuronal expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..77)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(99..248)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(273..293)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(983..1009)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aacagctcct cgcccttgct cactgggcca ggattctcct cgacgtcacc gcatgttagc
       61 agacttcctc tgccctctcc actgcctcta gagttaatca gcatgtccag gtcgaaatca
      121 tcaagggcgt cactgccgag catgtcgagg tcaaagtcat caagggcatc cgaaccaagc
      181 atgtcaaggt caaaatcatc gagggcgtca cttcccagca tatccagatc aaaatcgtcc
      241 aatgcgtcag cccgtccgga accgctggcc tccacctttc tcttcttctt ggggctagct
      301 gccactcgat gtgatgtcct ctcgggaatc cttctgttgg tccgcttaat caatgcaggc
      361 gcgtgcggga gccccttctt caccgcatcc aaggcgggtc gtccgccgag gcaggccagc
      421 gctacaagat gatcattagt gagtgcggcc agcgcggggt ccggcctcga aagctgggcc
      481 acgattgact ccagtgcggg tctgcccccg tcatgggatg caatagccac tacctgctca
      541 ggcgtgagtc gagacccgta cgggtctctg tcaggttgag gggtgctccc gtgagcgcat
      601 tgcgccacgc gtgcaccgcc tcgaccgccg tgactcctcc ccgcttcgcg atcttcagca
      661 actggcccgt gtccagctga aggggaggcc ctctcagctc tcccgcgacc gtcaacaggg
      721 cctcaagcgc tcgggctccg ctccactgtt tccccacacc gacgatcgcc tcatgtgtgg
      781 cttccggcaa cgccgcaatc atgtcctggt acttgacggc gaccgtgcca agggctgcag
      841 ggtgctgcga caaggctacg atgtgggcat gtgtaaaccc atgacccacc agcgcttcgt
      901 gatgctgcgc gactgtcgac ctcactttgg gcttgatctt ttcctgctgc tgctgtgaat
      961 atcccaaagt tctcaaatct acagcgtaat ctggaacatc gtatgggtac atggtggcgg
     1021 atccttctcg a
//
