LOCUS       sequence-72033-e        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal 
            alpha-importin nuclear localization sequence. EF1-alpha promoter for
            ubiquitous mammalian expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: LITE2.0
            EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2...
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..84
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tttcaggtgt cgtgaggtac cggatccgcc accatgaaaa ggccggcggc cacgaaaaag
       61 gccggccagg caaaaaagaa aaagaagatg gacaaaaaga cgattgtgtg gtttagacgt
      121 gaccttcgca ttgaggacaa ccctgcgttg gcagcggcag cgcatgaagg ttcggtattc
      181 cctgtcttta tctggtgtcc cgaagaggag gggcaattct atcccggtcg cgcgtcccga
      241 tggtggatga aacagtcgct cgcacatctt agccagtcgt tgaaggcgct cggctctgat
      301 ctgacgctca tcaaaacaca caatacaatt tctgccattc tggattgcat tagggtgact
      361 ggtgccacca aagttgtgtt caatcacctt tatgatcccg taagcctggt cagggatcat
      421 acagtaaaag agaaacttgt agagagggga atcagcgtcc aatcgtacaa tggggacttg
      481 ttgtatgaac cgtgggaaat ctattgtgag aaaggcaaac ctttcacttc cttcaattcg
      541 tactggaaga aatgcctcga catgtccatc gagtcagtca tgctgccgcc accttggaga
      601 cttatgccca ttactgctgc ggcagaggcg atctgggctt gcagcatcga agaacttggt
      661 ctggaaaacg aagcggaaaa acccagtaac gcgctcctga cgcgagcgtg gtcgccaggc
      721 tggtcgaatg ctgataagct ccttaatgag tttatcgaga aacagttgat cgactatgcg
      781 aagaatagca agaaggtggt gggaaattcc acttccctcc tgtcgccgta tctccacttt
      841 ggggagatta gcgtgaggca tgtgttccaa tgtgcgcgga tgaagcaaat catttgggct
      901 cgcgacaaaa actctgaagg agaggagtct gccgatttgt ttttgagagg aatcggcctt
      961 agggagtatt cacgatacat ctgcttcaac tttccgttta cacatgaaca atccctcctc
     1021 agccacctcc gcttctttcc gtggga
//
