LOCUS       sequence-72044-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length ETRAMP10.2 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: ETRAMP10.2-bio
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      182..191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(214..233)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(714..738)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(735..834)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(764..784)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccctcactct tataggccgt gatggaggtc gacggcgcgc cccgcttccg gttcttcttc
       61 tgctgcagat cctgctcgat ggccttcagg gccagcagat ttccccgggt ggtctggcca
      121 tttctggcgg ccgcaccaga catgcagagc agcagggaca tgagtacctg ggtctgaaac
      181 tccatggtgg caaacttgga cctgggagtg gacacctgtg gagagaaagg caaagtggat
      241 gtcattgtca ctcaagtgta tggccagatc gggccaggtg aatatcaaat cctcctcgtt
      301 tttggaaact gacaatctta gcgcagaagt aatgcccgct tttgagaggg agtactcacc
      361 ccaacagctg gatctcaagc ctgccacacc tcacctcgac catccgccgt ctcaagaccg
      421 cctactttaa ttacatcatc agcagcacct ccgccagaaa caaccccgac cgccacccgc
      481 tgccgcccgc cacggtgctc agcctacctt gcgactgtga ctggttagac gcctttctcg
      541 agaggttttc cgatccggtc gatgcggact cgctcaggtc cctcggtggc ggagtaccgt
      601 tcggaggccg acgggtttcc gatccaagag tactggaaag accgcgaaga gtttgtcctc
      661 aaccgcgagc ccaacagctg gccctcgcag acagcgatgc ggaagagagt gaggatctga
      721 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
      781 tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa
      841 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
      901 tatccacgcc cattggtgta ctgccaa
//
