LOCUS       sequence-72049-e         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a 
            T7 promoter for in vitro transcription, germline-compatible 5'UTR, 
            tbb-2 3'UTR and a polyA sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Katic I, Grosshans H
  TITLE     Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979578
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pIK86
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             172..192
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             199..219
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aatctcggag ctccagccgc tttcaagtac ttcgatacca caatcgatag aaagcgatat
       61 acttccacca aagaagttct ggatgcaaca cttattcatc aatcaatcac tggactgtat
      121 gaaactcgta ttgatttgtc gcaactcgga ggagatggaa gtggatcgat gccaaagaag
      181 aagcgtaagg tcgaagatcc aaaaaaaaaa agaaaagttg attagatgca aaatcctttc
      241 aagcattccc ttcttctcta tcactcttct ttctttttgt caaaaaattc tctcgctaat
      301 ttatttgctt ttttaatgtt attattttat gactttttat agtcactgaa aagtttgcat
      361 ctgagtgaag tgaatgctat caaaatgtga ttctgtctga tgtactttca caatctctct
      421 tcaattccat tttgaagtgc tttaaacccg aaaggttgag aaaaatgcga gcaaaaaaaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag cgctagcgct tttttttttt
      541 tttttttttt ttttttt
//
