LOCUS       sequence-72064-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate Compatible TALEN Construct for site specific 
            modification of genome, +63 C Terminus, Codon Optimized FokI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  TITLE     Bao Lab Golden Gate plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TALEN_C63
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..599)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    785..806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(821..826,827..844,845..851)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    859..875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     864..886
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     883..899
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     883..899
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     918..938
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        920..938
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(985..1004)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(986..1004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1014..1031)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1014..1030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1023..1045)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttaagttatc agaagttgat ctcgccgttg ttgaacttgc gccgcacctc ctccagtgtc
       61 agggtgccgg ctttgatcat ctcgccgccg atcagcagct cctccacgct cagcacggcg
      121 ccattgcagt tggtgatgtg gttcagcctg gtcagctggg ccttgtagtt gcccttgaag
      181 tggccgctca cgaacaggaa cttgaactcg gtcacgctgc tagggtacac cttccaccac
      241 tcgttggggt tgatgtgctt attccgggtc tggttctcct ccacgtatct ctgcatctcg
      301 tcggcctggc cgataggcag attgtagccg ccgctgtagg cctttgtgtc cacgatcacg
      361 ccgtaatcga tggggctgcc cactgtatag atggcgccgt caggctttct gcttccgccc
      421 aggtgctttc ccctgtagcc gtacaccttc atgaagaact ccatcacctt catctccagg
      481 atgcggtcct gggtgctgtt cctggcgatc tcgatcagct cgatgtactc gtggggcacg
      541 tacttcagct tgtgccgcag ctcggacttc ttctcctcca gctcgctctt caccagctgg
      601 gatcccgcga ctcgatggga agttctctcg ggaatccgcc ggttggttct tttgatcaat
      661 gcgggagcat gaggcagacc ctttttgact gcatcgagcg cgggtcgtcc accaagacat
      721 gccaacgcca ccagatggtc gttggagacg accggtggaa agcgggcagt gagcgcaacg
      781 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
      841 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
      901 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gaggacctcg
      961 agggggggcc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     1021 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1081 g
//
