LOCUS       sequence-72068-e        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate Compatible TALE Transcription Factor using VP64 Domain 
            with T2A-GFP addition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  TITLE     Bao Lab Golden Gate plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALE64G
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(73..93)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(73..91)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(112..128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(125..147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    136..152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(160..166,167..184,185..190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             757..777
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             802..951
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             973..1026
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(1069..1090)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcctc
       61 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
      121 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      181 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      241 actgcccgct ttccaccggt cgtctccaac gaccatctgg tggcgttggc atgtcttggt
      301 ggacgacccg cgctcgatgc agtcaaaaag ggtctgcctc atgctcccgc attgatcaaa
      361 agaaccaacc ggcggattcc cgagagaact tcccatcgag tcgcggatca cgcgcaagtg
      421 gtccgcgtgc tcggattctt ccagtgtcac tcccaccccg cacaagcgtt cgatgacgcc
      481 atgactcaat ttggtatgtc gagacacgga ctgctgcagc tctttcgtag agtcggtgtc
      541 acagaactcg aggcccgctc gggcacactg cctcccgcct cccagcggtg ggacaggatt
      601 ctccaagcga gcggtatgaa acgcgcgaag ccttcaccta cgtcaactca gacacctgac
      661 caggcgagcc ttcatgcgtt cgcagactcg ctggagaggg atttggacgc gccctcgccc
      721 atgcatgaag gggaccaaac tcgcgcgtca gctagcccca agaagaagag aaaggtggag
      781 gccagcggtt ccggacgggc tgacgcattg gacgattttg atctggatat gctgggaagt
      841 gacgccctcg atgattttga ccttgacatg cttggttcgg atgcccttga tgactttgac
      901 ctcgacatgc tcggcagtga cgcccttgat gatttcgacc tggacatgct gattaactct
      961 agaggcagtg gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct
     1021 ggcccagtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1081 gacggcgacg taaacggcca caagttca
//
