LOCUS       sequence-72069-e        1126 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate Compatible TALE Transcription Factor using VP64 Domain 
            with T2A-GFP addition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1126)
  TITLE     Bao Lab Golden Gate plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALE64G
FEATURES             Location/Qualifiers
     source          1..1126
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..83)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(105..254)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(279..299)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    830..851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(866..871,872..889,890..896)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    904..920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     909..931
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     928..944
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     928..944
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     963..983
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        965..983
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1030..1049)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1031..1049)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1059..1076)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1059..1075)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1068..1090)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcact gggccaggat tctcctcgac gtcaccgcat
       61 gttagcagac ttcctctgcc ctctccactg cctctagagt taatcagcat gtccaggtcg
      121 aaatcatcaa gggcgtcact gccgagcatg tcgaggtcaa agtcatcaag ggcatccgaa
      181 ccaagcatgt caaggtcaaa atcatcgagg gcgtcacttc ccagcatatc cagatcaaaa
      241 tcgtccaatg cgtcagcccg tccggaaccg ctggcctcca cctttctctt cttcttgggg
      301 ctagctgacg cgcgagtttg gtccccttca tgcatgggcg agggcgcgtc caaatccctc
      361 tccagcgagt ctgcgaacgc atgaaggctc gcctggtcag gtgtctgagt tgacgtaggt
      421 gaaggcttcg cgcgtttcat accgctcgct tggagaatcc tgtcccaccg ctgggaggcg
      481 ggaggcagtg tgcccgagcg ggcctcgagt tctgtgacac cgactctacg aaagagctgc
      541 agcagtccgt gtctcgacat accaaattga gtcatggcgt catcgaacgc ttgtgcgggg
      601 tgggagtgac actggaagaa tccgagcacg cggaccactt gcgcgtgatc cgcgactcga
      661 tgggaagttc tctcgggaat ccgccggttg gttcttttga tcaatgcggg agcatgaggc
      721 agaccctttt tgactgcatc gagcgcgggt cgtccaccaa gacatgccaa cgccaccaga
      781 tggtcgttgg agacgaccgg tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      841 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      901 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      961 gcgcaattaa ccctcactaa agggaacaaa agctggagga cctcgagggg gggcccggta
     1021 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     1081 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcag
//
