LOCUS       sequence-72079-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSP4 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSP4-bio
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      771..780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(803..822)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 ctcactctta taggccgtga tggaggtcga cggcgcgcca gagctgctgg ccagggacag
       61 cagctccacg cactcgatgc cttccagctt gtagccctct ttgcacttgc acttcacgcg
      121 ccggttgccc acgtactcgc acagcttatc gtcgccgcag ccgccgttgt tgtgcttgca
      181 caggtcctca tcttccaggt cgtccttgtt gtaggtgtca tcgtcctcat cgtcgtcgtc
      241 ctcttcgtgt tcctcttctt cctcgtgctc gccctcgccc tcgttgtggt cctcttcctc
      301 gcccacgtgg ccgccagaat tgctgctgct gggctgcacc actttcttgg ggatggcctc
      361 ggttttcttc ttgtcgtcca ccatctggct ttctttgggg ctcttttcca ggaccttttc
      421 ctgcaccttg tcggtcttgc cgtcatcttt cttctcgttg gttttctttt cctctttcac
      481 gtcgtccttg ccgttggcgc tttcctggct gttctctttc ttgtggcttt cctcgccctg
      541 ctcgctggcc tctttctcgt ccttcttctc ggcggcgtcg gccagattag ggctggcgct
      601 gctggcctca ttgccgccag gggtgttgct ggtgctcacg ccgtccacgt tgggcttctc
      661 ttcgcccagg atccgcatgt tcagcatccg gccgttctcg ggcacgatgt tgtaggcggc
      721 cgcaccagac atgcagagca gcagggacat gagtacctgg gtctgaaact ccatggtggc
      781 aaacttggac ctgggagtgg acacctgtgg agagaaaggc aaagtggatg tcattgtcac
      841 tcaagtgtat ggccagatcg ggccaggtga atatcaaatc ctcctcgttt ttgga
//
