LOCUS       sequence-72084-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T1ER is a calcium sensor targeted to ER.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Bandara S, Malmersjo S, Meyer T
  TITLE     Regulators of Calcium Homeostasis Identified by Inference of Kinetic
            Model Parameters from Live Single Cells Perturbed by siRNA.
  JOURNAL   Sci Signal. 2013 Jul 9;6(283):ra56. doi: 10.1126/scisignal.2003649.
  PUBMED    23838183
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-07-9"; volume: "6(283):ra56. doi"; pages: " 
            10.1126/scisignal.2003649"
COMMENT     Sequence Label: pcDNA-T1ER
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(106..127)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(262..774,775..777,778..780))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     455..474
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     714..735
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatacctgca
       61 gaattcttac agctcgtcct tgccgagagt gatcccggcg gcggtcacga actccagcag
      121 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt agctcaggta
      181 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      241 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
      301 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
      361 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      421 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      481 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      541 cttcatgtgg tcggggtagc gggcgaagca catcaggccg tagccgaagg tggtcacgag
      601 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
      661 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
      721 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
      781 gagctccagt gccccggagc tggagatctt ctcgaactcg ttggcagcgc tgacggcaat
      841 gaagtttttc tcccactccc tcttcccccc ctttgctgtc atcatttgta caa
//
