LOCUS       sequence-72097-e        6936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  adeno-associated virus (AAV) encoding a green fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Kaspar BK, Vissel B, Bengoechea T, Crone S, Randolph-Moore L, Muller
            R, Brandon EP, Schaffer D, Verma IM, Lee KF, Heinemann SF, Gage FH
  TITLE     Adeno-associated virus effectively mediates conditional gene 
            modification in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2320-5. Epub 2002 Feb 
            12.
  PUBMED    11842206
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-02-19"; volume: "99(4)"; pages: 
            "2320-5. Epub 2002 Feb 12"
COMMENT     Sequence Label: AAV GFP
FEATURES             Location/Qualifiers
     source          1..6936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     primer_bind     complement(198..217)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        319..698
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        699..902
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     853..873
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     899..923
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1036..1511
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      1574..1583
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1580..1582,1583..1585,1586..2296)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1580..1582,1583..1585,1586..2296)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1625..1646)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1886..1905)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2233..2254
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2421..2815
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      3720..4233
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3807..3826)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4017..4038
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4714..4733)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4833..4855
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4893..4911)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4979..5083
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5084..5152,5153..5944)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5302..5321)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6115..6703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6604..6623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccggcct cggcctctgc ataaataaaa aaaattagtc agccatgagc
      241 ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
      301 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      361 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      421 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      481 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggactattta cggtaaactg
      541 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      601 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      661 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      721 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      781 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      841 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      901 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      961 gaagacaccg ggaccgatcc agcctcccct cgaagcttac atgtggtacc gagctcggat
     1021 cctgagaact tcagggtgag tctatgggac ccttgatgtt ttctttcccc ttcttttcta
     1081 tggttaagtt catgtcatag gaaggggaga agtaacaggg tacacatatt gaccaaatca
     1141 gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt
     1201 tatcttattt ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta
     1261 tcatgcctct ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag
     1321 caatatttct gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat
     1381 attgctaata gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag
     1441 gctggattat tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct
     1501 tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag
     1561 cacgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1621 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1681 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1741 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     1801 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1861 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1921 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1981 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     2041 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     2101 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2161 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     2221 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2281 ggacgagctg tacaagtccg gactcagatc ctactagtag gatctcgagc tcaagcttcg
     2341 aattcacccc accagtgcag gctgcctatc agaaagtggt ggctggtgtg gctaatgccc
     2401 tggcccacaa gtatcactaa gctcgctttc ttgctgtcca atttctatta aaggttcctt
     2461 tgttccctaa gtccaactac taaactgggg gatattatga agggccttga gcatctggat
     2521 tctgcctaat aaaaaacatt tattttcatt gcaatgatgt atttaaatta tttctgaata
     2581 ttttactaaa aagggaatgt gggaggtcag tgcatttaaa acataaagaa atgaagagct
     2641 agttcaaacc ttgggaaaat acactatatc ttaaactcca tgaaagaagg tgaggctgca
     2701 aacagctaat gcacattggc aacagcccct gatgcctatg ccttattcat ccctcagaaa
     2761 aggattcaag tagaggcttg atttggaggt taaagttttg ctatgctgta ttttacatta
     2821 cttattgatt tagctgcctc atgaatgtct tttcactacc catttgctta tcctgcatct
     2881 ctcagccttg actccactca gttctcttgc ttagagatac cacctttccc ctgaagtgtt
     2941 ccttccatgt tttacggcga gatggtttct cctcgcctgg ccactcagct tagttgtctc
     3001 tgttgtctta tagaggtcta cttgaagaag gaaaaacagg gggcatggtt tgactgtcct
     3061 gtgagccctt cttcctgcct cccccactca cagtgacccg gaatccctcg acatggcagt
     3121 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     3181 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     3241 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     3301 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3361 gaatggcgaa tggaattcca gacgattgag cgtcaaaatg taggtatttc catgagcgtt
     3421 tttcctgttg caatggctgg cggtaatatt gttctggata ttaccagcaa ggccgatagt
     3481 ttgagttctt ctactcaggc aagtgatgtt attactaatc aaagaagtat tgcgacaacg
     3541 gttaatttgc gtgatggaca gactctttta ctcggtggcc tcactgatta taaaaacact
     3601 tctcaggatt ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc
     3661 tcccgctctg attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc aaccatagta
     3721 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3781 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3841 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3901 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3961 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4021 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4081 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4141 cgcgaatttt aacaaaatat taacgtttac aatttaaata tttgcttata caatcttcct
     4201 gtttttgggg cttttctgat tatcaaccgg ggtacatatg attgacatgc tagttttacg
     4261 attaccgttc atcgattctc ttgtttgctc cagactctca ggcaatgacc tgatagcctt
     4321 tgtagagacc tctcaaaaat agctaccctc tccggcatga atttatcagc tagaacggtt
     4381 gaatatcata ttgatggtga tttgactgtc tccggccttt ctcacccgtt tgaatcttta
     4441 cctacacatt actcaggcat tgcatttaaa atatatgagg gttctaaaaa tttttatcct
     4501 tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg gtcataatgt ttttggtaca
     4561 accgatttag ctttatgctc tgaggcttta ttgcttaatt ttgctaattc tttgccttgc
     4621 ctgtatgatt tattggatgt tggaattcct gatgcggtat tttctcctta cgcatctgtg
     4681 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4741 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4801 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4861 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     4921 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4981 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5041 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5101 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5161 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5221 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5281 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5341 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5401 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5461 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5521 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5581 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5641 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5701 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5761 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5821 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5881 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5941 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6001 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6061 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6121 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6181 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6241 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6301 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6361 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6421 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6481 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6541 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6601 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6661 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6721 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6781 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6841 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6901 accgcctctc cccgcgcgtt ggccgattca ttaatg
//
