LOCUS       sequence-72098-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia with mCherry 
            reference.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-mCherry-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(163..606)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     722..741
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat
      121 ccggtggatc ccgggcccgc ggtaccgtcg actgcagaat tcttacttcg ctgtcatcat
      181 ttgtacaaac tcttcgtagt ttacctgacc atccccatcg atgtctgcta ccctgatcat
      241 ttcatcaacc tcttcatctg ttaacttctc tccaaggttt gtcatcacgt ggcgaagctc
      301 tgctgcgccg atgtagccat tgccgtcctt atcaaacaca cggaacgctt ctctaatttc
      361 ttcttcactg tctgtgtctt tcatttttct tgccatcatt gtcaggaact cagggaagtc
      421 gattgtgccg tcaccgtcgg catctacttc attgatcatg tcctgcagct ctgcttctgt
      481 ggggttctgc cccagagacc gcatcaccgt ccccagctcc ttggttgtta tcgtcccatc
      541 cccgtccttg tcaaataggg agaaagcctc tttaaattct gcgatctgct cttcagtcag
      601 ttggtcacgc gtgttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      661 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      721 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggatgt agccttcggg
//
