LOCUS       sequence-72102-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for pH inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-CFP-Venus(H148G)
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(134..385)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(176..197)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     525..544
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
       61 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt tatctagatc
      121 cggtggatcc ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      181 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt
      241 agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      301 ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg
      361 ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgccgctg ttgtagttgt
      421 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      481 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      541 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      601 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcaggccg tagcccaggg
      661 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatc agcttcaggg
      721 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      781 ttacgtcgcc gt
//
