LOCUS       sequence-72104-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-GCaMP5G
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(66..87)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(300..356)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      368..377
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(384..398,399..407))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(411..443)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(447..464)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accatgctcc ctccggtacc gcccttgtac agctcgtcca tgccgagagt gatcccggcg
       61 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttcgaaagt
      121 ttggactgca cgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      181 gtgttctgct ggtagtggta ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc
      241 ttgaagttcg ccttgatgcc gttcttctgc ttgtcggcct tgatatagac gttctcgagt
      301 gagctcagcc gacctatagc tctgactgcg tgacctgtct tattccactt acgacgtgat
      361 gagtcgacca tggtggcgag atccttatcg tcatcgtcgt acagatcccg acccatttgc
      421 tgtccaccag tcatgctagc cataccatga tgatgatgat gatgagaacc cattcctcct
      481 gcgctaccac cagcactgga tcccgggccc gcggtaccgt tcatggggga accaagtgga
      541 tgctgccgta tctcaggctc cacagagtct gtgacgaaga agttgaccac agtaggggcc
      601 ctggtgggtg gcggggggtc ccgggtcgcc tctccaggca gcaaaagctg ggctgtgagc
      661 tgcaggcccg aggtgccccc tgaagctgag tctgaatctg agttgggtgg cagaggcagg
      721 ggcagcacct gctgcaggct gaggcctggc ctggcaccac tgtgagaggt ccacatg
//
