LOCUS       sequence-72106-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV/TO 
            promoter, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV/TO Hygro DEST (693-2)
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      34..169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    209..330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(220..239)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     274..293
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             402..635
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gactcactat agggcgaatt gggtacgtcc ctaggcctcc aaaaaagcct cctcactact
       61 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
      121 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
      181 ggggcgggat agctagagcc agacatgata agatacattg atgagtttgg acaaaccaca
      241 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
      301 gtaaccatta taagctgcaa taaacaagtt cctctcactc tctgatattc atttctttgc
      361 aagttataaa tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac
      421 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
      481 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
      541 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag
      601 atcttgtctt cgttgggagt gagctagccc ttccagtccc cccttttctt ttaaaaagtg
      661 gctaagatct acagctgcct tgtaagtcat tggtcttaaa ggtaccgggc cctcgacgga
      721 tctgcaccca atcggcaggc acgggcggcg atctccaatc tgcgggatca gtcagatcac
      781 ccgagtgcgt gggcatgaca atcgtgccct ggggaccaac acaatccaga agggcctgaa
      841 tcactgcgac cgg
//
