LOCUS       sequence-72108-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-GCaMP5G
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(155..598)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     714..733
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat
      121 ccggtggatc tgtcgactgc agagttcgaa gctttcactt cgctgtcatc atttgtacaa
      181 actcttcgta gtttacctga ccatccccat cgatgtctgc ttccctgatc atttcatcaa
      241 cctcttcatc tgttaacttc tctccaaggt ttgtcatcac gtggcgaagc tctgctgcac
      301 tgatgtagcc attgccatcc ttatcaaaca cacggaacgc ttctctaatt tcttcttcac
      361 tgtctgtgta tttcattttt cttgccatca ttgtcaggaa ctcagggaag tcgattgtgc
      421 cgtcaccgtc ggcatctact tcattgatca tgtcctgcag ctctgcttct gtggggttct
      481 gccccagaga ccgcatcacc gtccccagct ccttggttgt tattgtccca tccccgtcct
      541 tgtcaaatag ggagaaagcc tctttaaatt ctgcgatctg ctcttcagtc agttggtccg
      601 gcaggttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
      661 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
      721 agttgccgtc gtccttgaag aagatggtgc gctcctggat gtagccttcg ggcatggcgg
      781 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcac
//
