LOCUS       sequence-72112-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for pH inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-CFP-Venus(H148G)
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgaaccgtca gatccgctag cgccaccatg gttccagagc ccggccctgt caacagtagc
       61 accccagcct ggggccccgg gccaccgcca gccccagggg gcagcggctg ggtggccgct
      121 gcgctgtgcg tggtcatcgt gctgacggca gccgccaatt cgctgctgat cgcgctcatc
      181 tgcacgcagc ccgcgctgcg caacacgtct aacttcttcc tggtgtcgct cttcacgtcg
      241 gacctgatgg tgggattggt ggtgatgccc ccagccatgc tgaacgcgct gtatgggcgc
      301 tgggtactag ctcgcggcct ctgtctgctt tggaccgcct tcgacgtgat gtgctgcagc
      361 gcctccattc tcaacctctg cctcatcagc ctggaccgct acctgctcat cctctcgccg
      421 ctgcgctaca agctgcgcat gacagccccg cgtgccctgg cgctcatcct gggtgcctgg
      481 agcctcgccg cgctggcctc cttcctgccc ctcttgctgg gctggcatga actgggcaaa
      541 gctcgaacat ctgccccggg ccagtgccgc ctattggcca gcctgcctta tgtcctcgtg
      601 gcgtccggcg tcaccttttt cctgccttcg ggtgccatct gcttcaccta ctgcaggatc
      661 ttgctggctg cccgcaagca ggcggtgcaa gtggcctcgc tcaccacggg cacggctacg
      721 gctggccagg ccttggaaac cttgcaggtg cccaggacac cacgcccagg gatggagtct
      781 gct
//
