LOCUS       sequence-72115-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..470)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     586..605
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cagtgtgatg gatatctgca gaattcttac ttcgctgtca tcatttgtac aaactcttcg
       61 tagtttacct gaccatcccc atcgatgtct gctaccctga tcatttcatc aacctcttca
      121 tctgttaact tctctccaag gtttgtcatc acgtggcgaa gctctgctgc gccgatgtag
      181 ccattgccgt ccttatcaaa cacacggaac gcttctctaa tttcttcttc actgtctgtg
      241 tctttcattt ttcttgccat cattgtcagg aactcaggga agtcgattgt gccgtcaccg
      301 tcggcatcta cttcattgat catgtcctgc agctctgctt ctgtggggtt ctgccccaga
      361 gaccgcatca ccgtccccag ctccttggtt gttatcgtcc catccccgtc cttgtcaaat
      421 agggagaaag cctctttaaa ttctgcgatc tgctcttcag tcagttggtc acgcgtgttg
      481 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      541 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      601 tcgtccttga agaagatggt gcgctcctgg atgtagcctt cgggcatggc ggacttgaag
      661 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
      721 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
      781 gtcagcttgc cgta
//
