LOCUS       sequence-72117-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia with mCherry 
            reference.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-mCherry-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(327..346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc
       61 ccatccaggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      121 gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca
      301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      421 acggcaacat cctggggcac aagctggagt acaacacgcg tgaccaactg actgaagagc
      481 agatcgcaga atttaaagag gctttctccc tatttgacaa ggacggggat gggacgataa
      541 caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca gaagcagagc
      601 tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac ttccctgagt
      661 tcctgacaat gatggcaaga aaaatgaaag acacagacag tgaagaagaa attagagaag
      721 cgttccgtgt gtttgataag gacggcaatg gctacatcgg cgcagcagag cttcgccacg
      781 tgatgaca
//
