LOCUS       sequence-72118-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for Ca2+ inside primary cilia with mCherry 
            reference.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: 5HT6-mCherry-G-GECO1.0
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             260..316
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     529..550
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
       61 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
      121 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
      181 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtccg gactcagatc
      241 caccatggtc gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat
      301 aggtcggctg agctcactcg agaacgtcta tatcaaggcc gacaagcaga agaacggcat
      361 caaggcgaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
      421 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      481 gagcgtgcag tccatacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
      541 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
      601 cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatccaggt
      661 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
      721 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc t
//
