LOCUS       sequence-72122-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pCP5
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(487..718)
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(735..752)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(735..751)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcaggtcga tacagtagaa attacagaaa ctttatcacg tttagtaagt atagaggctg
      181 aaaatccaga tgaagccgaa cgacttgtaa gagaaaagta taagagttgt gaaattgttc
      241 ttgatgcaga tgattttcag gactatgaca ctagcgtata tgaataggta gatgttttta
      301 ttttgtcaca caaaaaagag gctcgcacct ctttttctta tttcttttta tgatttaata
      361 cggcattgag gacaatagcg agtaggctgg atacgacgat tccgtttgag aagaacattt
      421 ggaaggctgt cggtcgacta agttggcagc atcacccgaa gaacatttgg aaggctgtcg
      481 gtcgactaca ggtcactaat accatctaag tagttgattc atagtgactg gatatgttgt
      541 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      601 atatcatttt acgtttctcg ttcagctttt ttgtacaaag ttggcattat aaaaaagcat
      661 tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgg
      721 ggcccgagct taagactggc cgtcgtttta caacagatct ctgataccat gaaacccatt
      781 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      841 cgcatagggt aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca
      901 tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc
      961 ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaa
//
