LOCUS       sequence-72129-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  TITLE     Integrated Genomics
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLexAgtwy
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..135
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(160..165,166..183,184..190)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(310..329)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 attcccatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
       61 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
      121 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
      181 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
      241 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
      301 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
      361 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
      421 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
      481 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
      541 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
      601 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
      661 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
      721 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
      781 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
      841 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atga
//
