LOCUS       sequence-72130-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pCP5
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        125..272
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             273..947
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 ctatatatat aatataggaa gcatttaata gaacagcatc gtaatatatg tgtactttgc
       61 agttatgacg ccagatggca gtagtggaag atattcttta ttgaaaaata gcttgtcacc
      121 ttacgtacaa tcttgatccg gagcttttct ttttttgccg attaagaatt aattcggtcg
      181 aaaaaagaaa aggagagggc caagagggag ggcattggtg actattgagc acgtgagtat
      241 acgtgattaa gcacacaaag gcagcttgga gtatgtctgt tattaatttc acaggtagtt
      301 ctggtccatt ggtgaaagtt tgcggcttgc agagcacaga ggccgcagaa tgtgctctag
      361 attccgatgc tgacttgctg ggtattatat gtgtgcccaa tagaaagaga acaattgacc
      421 cggttattgc aaggaaaatt tcaagtcttg taaaagcata taaaaatagt tcaggcactc
      481 cgaaatactt ggttggcgtg tttcgtaatc aacctaagga ggatgttttg gctctggtca
      541 atgattacgg cattgatatc gtccaactgc atggagatga gtcgtggcaa gaataccaag
      601 agttcctcgg tttgccagtt attaaaagac tcgtatttcc aaaagactgc aacatactac
      661 tcagtgcagc ttcacagaaa cctcattcgt ttattccctt gtttgattca gaagcaggtg
      721 ggacaggtga acttttggat tggaactcga tttctgactg ggttggaagg caagagagcc
      781 ccgaaagctt acattttatg ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc
      841 ttagattaaa tggcgttatt ggtgttgatg taagcggagg tgtggagaca aatggtgtaa
      901 aagactctaa caaaatagca aatttcgtca aaaatgctaa gaaataggtt attactgagt
      961 agtatttatt taagtattgt ttgtgcactt gccgatctat gcggtgtgaa ataccgcaca
     1021 gatgcgtaag gaga
//
