LOCUS       sequence-72134-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV/TO 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV/TO DEST (775-1)
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    79..203
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(228..233,234..251,252..258)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(378..397)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgactagt ccagtgtggt
       61 ggaattctgc agatatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
      121 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
      181 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg
      241 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
      301 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      361 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      421 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      481 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
      541 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      601 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
      661 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
      721 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
      781 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
      841 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtt
//
