LOCUS       sequence-72135-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Marx CJ, Lidstrom ME
  TITLE     Development of improved versatile broad-host-range vectors for use 
            in methylotrophs and other Gram-negative bacteria.
  JOURNAL   Microbiology. 2001 Aug;147(Pt 8):2065-75.
  PUBMED    11495985
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2001-08"; volume: "147(Pt 8)"; pages: 
            "2065-75"
COMMENT     Sequence Label: pCM66
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..27
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     9..26
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    34..90
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(92..111)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(93..111)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(118..135)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(118..134)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(127..149)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      275..730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(362..381)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     572..593
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(797..816)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     916..938
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(976..994)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gccaagctat ttaggtgaca ctatagaata ctcaagcttg catgcctgca ggtcgactct
       61 agaggatccc cgggtaccga gctcgaattc gccctatagt gagtcgtatt acaattcact
      121 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      181 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      241 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
      301 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      361 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      421 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
      481 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
      541 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
      601 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
      661 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
      721 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      781 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
      841 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
      901 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
      961 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atga
//
