LOCUS       sequence-72138-e        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pCP5
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             391..696
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     575..594
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttaatgaatt acaacagtac tgcgatgagt ggcagggggg gcgtaaacgc cgcgtggatc
       61 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
      121 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt
      181 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
      241 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
      301 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
      361 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga
      421 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
      481 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
      541 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
      601 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
      661 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
      721 cagtctgcag gtcgatacag tagaaattac agaaacttta tcacgtttag taagtataga
      781 ggctgaaaat ccagatgaag ccgaacgact tgtaagagaa aagtataaga gttgtgaaat
      841 tgttcttgat gcagatgatt ttcaggacta tgacactagc gtatatgaat aggtagatgt
      901 ttttattttg tcacacaaaa aagaggctcg cacctctttt tcttatttct ttttatgatt
      961 taatacggca ttgaggacaa tagcgagtag gctggatacg acgattccgt ttgagaagaa
     1021 catttggaag gctgtcggtc gactaagttg gcagcatcac ccgaagaaca tttggaaggc
     1081 tgtcg
//
