LOCUS       sequence-72142-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation Lentiviral vector for Tet-based inducible shRNA or 
            cDNA expression, constitutive Neomycin resistance gene 
            coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pSLIK-Neo
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(308..327)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ttccaaattt tgtaatccag aggttgatta tcgataagct tgatatcgaa ttgggaggga
       61 ctaaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
      121 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg
      181 ccgctttact tgtacatcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
      241 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
      301 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
      361 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
      421 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
      481 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
      541 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
      601 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
      661 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
      721 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
      781 ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg
      841 tcttgacaaa
//
