LOCUS       sequence-72143-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for Tet-based inducible shRNA or cDNA expression, 
            constitutive Zeocin resistance gene coexpression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, 
            Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
  TITLE     A single lentiviral vector platform for microRNA-based conditional 
            RNA interference and coordinated transgene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
  PUBMED    16945906
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Sep 12. 103(37):13759-64."
COMMENT     Sequence Label: pSLIK-Zeo
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(191..565)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(584..631)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 taatccagag gttgattatc gataagcttg atatcgaatt gggagggact aaacaacaac
       61 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
      121 taaaacctct acaaatgtgg tatggctgat tatgatctag agtcgcggcc gctttacttg
      181 tacagatgtg tcagtcctgc tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc
      241 gcagggcgaa ctcccgcccc cacggctgct cgccgatctc ggtcatggcc ggcccggagg
      301 cgtcccggaa gttcgtggac acgacctccg accactcggc gtacagctcg tccaggccgc
      361 gcacccacac ccaggccagg gtgttgtccg gcaccacctg gtcctggacc gcgctgatga
      421 acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag tcccgggaga
      481 acccgagccg gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg
      541 gaacggcact ggtcaacttg gccatggttt agttcctcac cttgtcgtat tatactatgc
      601 cgatatacta tgccgatgat taattgtcaa cacatcacgc gtaccggtaa gcttatcatc
      661 gtgtttttca aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc
      721 tcgactaaac acatgtaaag catgtgcacc gaggccccag
//
