LOCUS       sequence-72154-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pCP5
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(23..41)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    407..454
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taattaataa
       61 tggtttctta gtatgatcca atatcaaagg aaatgatagc attgaaggat gagactaatc
      121 caattgagga gtggcagcat atagaacagc taaagggtag tgctgaagga agcatacgat
      181 accccgcatg gaatgggata atatcacagg aggtactaga ctacctttca tcctacataa
      241 atagacgcat ataagtacgc atttaagcat aaacacgcac tatgccgttc ttctcatgta
      301 tatatatata caggcaacac gcagatatag gtgcgacgtg aacagtgagc tgtatgtgcg
      361 cagctcgcgt tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag ttcctattcc
      421 gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc
      481 tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata
      541 ttgcgaatac cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg
      601 ctatatccct atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg
      661 acctctacat tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa
      721 gaactattca tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt
      781 atatagagac aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac
      841 gctatcactt tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg
      901 atgcctttat cttgaaaaaa tg
//
