LOCUS       sequence-72156-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to generate synthetic guide in vitro from T7 promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB60
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     141..158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     215..231
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        252..270
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_RNA        complement(288..363)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(408..425)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(408..424)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        672..776
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
       61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcattaggc accccaggct
      121 ttacccgaac gaccgagcgc agcgagtcag tgagcgagga agcggagagc gcccaatacg
      181 caaggaaaca gctatgacca tgttaatgca gctgcaggaa acagctatga ccatgattac
      241 gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tttaaaagca ccgactcggt
      301 gccacttttt caagttgata acggactagc cttattttaa cttgctattt ctagctctaa
      361 aactgagacc ctctctcggt ctcttatagt gagtcgtatt acaattcact ggccgtcgtt
      421 ttacagctgg cgtaatagcg aagaggcccg caccgaaacg cccttcccaa cagttgcgca
      481 gcctgaatgg cgaatgggag cgccctgtag cggccactca accctatctc ggtctattct
      541 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      601 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
      661 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      721 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      781 ttgaacagga tggcctgcat gcgggtagcc cgg
//
