LOCUS       sequence-72157-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to express a CRISPR guide RNA in C. elegans from U6 
            regulatory sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den
            Heuvel S, Boxem M
  TITLE     CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979586
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pMB70
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..66
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     50..66
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_RNA        267..342
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagaactag
       61 tggatccccc gggctgcagg aattcgatga gctcccaaca catagtgttt ccaatgttat
      121 acccaatcaa taatagcaag tcaataaact acctctacac tatttctggt aataggcgaa
      181 acctctacac tgtcagtcac tttgtaaggt gtgcctatat atttccataa tatttcatac
      241 aaattagaga ccgagagagg gtctcagttt tagagctaga aatagcaagt taaaataagg
      301 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttta tcaagcttct
      361 gacatagagt tttacatata tcttctctgt attgtttcaa ttttagtata tgttctcgaa
      421 agaaatactc ttaaaataat ctaattttac caagagccat aattcaacac ttcaaaaagt
      481 tagtagttgt tctaaattgt cagctacatt tttggcgaat tgccaaattt ctgaaaaaat
      541 agtttataga tttgttgcca actaatttct gaattttgat ctaccgtaat gcttataaca
      601 tatggagcgc cagtgggaaa actgttggaa acgctgtaat agtgctaaca tgaccaaaca
      661 acatagtgaa agagacacca cgaattcaag atctggccga attacggttt ccggatttga
      721 cactgttcca gatccggaaa tctaccagat cttgaatccg tggtgtctct agtgga
//
