LOCUS       sequence-72159-e        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV TALE synthesis backbone with HD half repeat; fused to nuclear 
            localization sequence linker and VP64 transcriptional activator 
            effector domain. Synapsin promoter for neuronal expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             431..457
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
       61 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
      121 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
      181 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
      241 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
      301 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
      361 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc
      421 cgccaccatg tacccatacg atgttccaga ttacgctgta gatttgagaa ctttgggata
      481 ttcacagcag cagcaggaaa agatcaagcc caaagtgagg tcgacagtcg cgcagcatca
      541 cgaagcgctg gtgggtcatg ggtttacaca tgcccacatc gtagccttgt cgcagcaccc
      601 tgcagccctt ggcacggtcg ccgtcaagta ccaggacatg attgcggcgt tgccggaagc
      661 cacacatgag gcgatcgtcg gtgtggggaa acagtggagc ggagcccgag cgcttgaggc
      721 cctgttgacg gtcgcgggag agctgagagg gcctcccctt cagctggaca cgggccagtt
      781 gctgaagatc gcgaagcggg gaggagtcac ggcggtcgag gcggtgcacg cgtggcgcaa
      841 tgcgctcacg ggagcacccc tcaacctgac agagacccgt acgggtctcg actcacgcct
      901 gagcaggtag tggctattgc atcccatgac gggggcagac ccgcactgga gtcaatcgtg
      961 gcccagcttt cgaggccgga ccccgcgctg gccgcactca ctaatgatca tcttgtagcg
     1021 ctggcctgcc tcggcggacg acccgccttg gatgcg
//
