LOCUS       sequence-72160-e        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tandem dual tomato plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, 
            Lowy DR, Schiller JT
  TITLE     Genital transmission of HPV in a mouse model is potentiated by 
            nonoxynol-9 and inhibited by carrageenan.
  JOURNAL   Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
  PUBMED    17603495
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med."; 
            date: "2007-07"; volume: "13(7)"; pages: "857-61. Epub 2007 Jul 1"
COMMENT     Sequence Label: ptwB
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(103..126)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(301..320)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     570..588
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      718..727
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(734..748,749..757))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     intron          807..1010
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(830..850)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cggtgccgga gctgccgctg ccggtgctgc cggtgccatg ccccaggaac aggtggtggc
       61 ggccctcgga gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt
      121 ccagcttggt gtccacgtag tagtagccgg gcagttgcac gggcttcttg gccatgtaga
      181 tggtcttgaa ctccaccagg tagtggccgc cgtccttcag cttcagggcc tggtggatct
      241 cgcccttcag cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc cagcccatgg
      301 tcttcttctg cattacgggg ccgtcggggg ggaagttggt gccgcgcatc ttcaccttgt
      361 agatcagcgt gccgtcctgc agggaggagt cctgggtcac ggtcaccaga ccgccgtcct
      421 cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc ttgtaatcgg
      481 ggatgtcggc ggggtgcttc acgtacgcct tggagccgta catgaactgg ggggacagga
      541 tgtcccaggc gaagggcagg gggccgccct tggtcacctt cagcttggcg gtctgggtgc
      601 cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcatggagc
      661 cctccatgcg caccttgaag cgcatgaact ctttgatgac ctcctcgccc ttgctcacca
      721 tggtggcggg atccttatcg tcatcgtcgt acagatcccg acccatttgc tgtccaccag
      781 tcatgctaga gaattcctca cgacacctga aatggaagaa aaaaactttg aaccactgtc
      841 tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa ggagaattac
      901 atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg aaactccatc
      961 gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga gaactaatcg
     1021 aggtgcctgg acggcgcccg gtactccgt
//
