LOCUS       sequence-72166-e        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dsRed plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, 
            Lowy DR, Schiller JT
  TITLE     Genital transmission of HPV in a mouse model is potentiated by 
            nonoxynol-9 and inhibited by carrageenan.
  JOURNAL   Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
  PUBMED    17603495
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med."; 
            date: "2007-07"; volume: "13(7)"; pages: "857-61. Epub 2007 Jul 1"
COMMENT     Sequence Label: p8RwB
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(97..771)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(156..179)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(354..373)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     571..591
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     623..641
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      768..777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(901..921)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tcctgcagcc cgggggatcc actagtgtcg agatctcagg aacaggtggt ggcggccctc
      121 ggcgcgctcg tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
      181 ggagtccacg tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt
      241 gaactccacc aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt
      301 cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tagtcttctt
      361 ctgcattacg gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa
      421 ggagccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
      481 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc
      541 ggcggggtgc ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca
      601 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
      661 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
      721 gcgcaccttg aagcgcatga actccttgat gacgtcctcg gaggaggcca tggtggcgac
      781 cggtagctcg actgcagaat tcgaagctta cttagatcgc agatccagca caatggatct
      841 cgaggtcgag ggatctctag agaattcctc acgacacctg aaatggaaga aaaaaacttt
      901 gaaccactgt ctgaggcttg agaatgaacc aagatccaaa ctcaaaaagg gcaaattcca
      961 aggagaatta catcaagtgc caagctggcc taacttcagt ctccacccac tcagtgtggg
     1021 gaaactccat cgcataaaac ccctcccccc aacctaaaga c
//
