LOCUS       sequence-72167-e        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dsRed plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Johnson KM, Kines RC, Roberts JN, Lowy DR, Schiller JT, Day PM
  TITLE     Role of heparan sulfate in attachment to and infection of the murine
            female genital tract by human papillomavirus.
  JOURNAL   J Virol. 2009 Mar;83(5):2067-74. Epub 2008 Dec 10.
  PUBMED    19073722
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2009-03"; volume: "83(5)"; pages: "2067-74. Epub 2008 Dec 10"
COMMENT     Sequence Label: pRwB
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      116..125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             122..796
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(252..270)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(302..322)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     520..539
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     714..737
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(908..928)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tttcttccat ttcaggtgtc gtgaggaatt ctctagagat ccctcgacct cgagatccat
       61 tgtgctggat ctgcgatcta agtaagcttc gaattctgca gtcgagctac cggtcgccac
      121 catggcctcc tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg
      181 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      241 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
      301 cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc
      361 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
      421 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggct ccttcatcta
      481 caaggtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaagac
      541 tatgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
      601 gatccacaag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat
      661 ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggact ccaagctgga
      721 catcacctcc cacaacgagg actacaccat cgtggagcag tacgagcgcg ccgagggccg
      781 ccaccacctg ttcctgagat ctcgacacta gtggatcccc cgggctgcag gaattcgata
      841 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
      901 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
      961 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     1021 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     1081 acgcaacccc cactggttgg ggcattgcca
//
