LOCUS       sequence-72190-e        9852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human NKCC1 mutant with HA tag inserted into extracellular
            loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is 
            synthetic, containing convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)
FEATURES             Location/Qualifiers
     source          1..9852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..96
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(109..111,112..822)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(109..111,112..822)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(151..172)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(412..431)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     759..780
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2548..2574
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2578..2604
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4552..4569)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4558..4782
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4828..5256
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4915..4934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5125..5146
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5265..5285)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5270..5599
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5450..5585
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5512..5531
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5666..6460
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5720..5739)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6330..6349
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6634..6755
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6671..6690)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6725..6744
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6804..6820)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6804..6820)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6817..6839)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6828..6844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6852..6858,6859..6876,6877..6882))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6897..6918
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7035..7052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7206..7791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7286..7305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7962..8753,8754..8822))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8585..8604
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8823..8927)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9002..9021)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9193..9572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9573..9776
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9727..9747
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9821..9840
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9821..9839
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctagcccgg gcacggccgc caccatggga gactacaaag accatgacgg tgattataaa
       61 gatcacgaca tcgactacaa ggatgacgat gacaagggtg accctaccgt cagcaagggc
      121 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      181 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      241 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      301 ggctacggcc tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      361 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      421 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      481 ctgaagggca tcgacttcaa ggaggacggc aacatcctag ggcacaagct ggagtacaac
      541 tacaacagcc acaacgttta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      601 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      661 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
      721 tccaaactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      781 accgccgccg ggatcactct cggcatggac gagctctaca agggtgacct ggagccgagg
      841 cccacggctc ctagctccgg cgcgccaggc ctcgccggag tcggcgaaac gccgagcgcc
      901 gctgcgctgg ccgcagccag ggtggaactg cccggaaccg ctgtgccctc ggtgccggag
      961 gatgctgcgc ccgcgagccg ggacgggggc ggggtccgcg atgagggacc agccgccgcc
     1021 ggtgacggcc tgggcagacc cttggggcct accccgagcc agagccgttt ccaggtggac
     1081 ctcgtttccg agaacgcagg gcgggctgct gctgcggcgg cggcggctgc tgcggccgcc
     1141 gccgctgctg gtgccggggc gggggccaag cagactcctg ccgacgggga agccagcggc
     1201 gagagcgagc cagctaaagg cagcgaggaa gccaaaggcc gcttccgcgt taacttcgta
     1261 gatcctgctg ccagctcgtc cgcagaagat agcctgtcag atgccgccgg ggtcggagtc
     1321 gatggaccca acgtgagctt ccagaacggc ggggacacgg tactgagcga gggcagcagc
     1381 ctccattccg gtggcggcgg cggctccggc caccaccagc actactatta tgatacccac
     1441 accaacacct actatctgcg caccttcggc cacaacacaa tggacgcggt acccaggatc
     1501 gaccactacc ggcacacagc cgcgcagctc ggcgagaagc tgctccggcc tagcctggcc
     1561 gagctgcatg acgagctgga aaaggaacct tttgaggatg gctttgcaaa tggggaagaa
     1621 agcactccaa cacgtgatgc tgtggtcacg tacacggccg aaagtaaagg agtcgtgaag
     1681 tttggctgga tcaagggtgt actagtacgt tgtatgttaa acatttgggg tgtgatgctt
     1741 ttcattcgtc tctcatggat tgtgggacaa gctggaattg gtctatcagt ccttgtaata
     1801 atgatggcta ctgttgtgac aactatcaca ggattgtcca ctagcgccat cgcaactaat
     1861 ggatttgtaa ggggaggcgg cgcatattat ttaatatcta gaagtctagg gcctgaattt
     1921 ggtggtgcca ttggtctaat cttcgccttt gccaacgctg ttgctgttgc tatgtatgtg
     1981 gttggatttg cagaaaccgt ggtggagttg ctgaaagaac attccattct tatgatagat
     2041 gaaatcaatg acatccggat tattggagcc attacagtcg tgattctttt aggtatctca
     2101 gtagccggta tggagtggga agcaaaagct cagattgttc ttttggtgat cctacttctt
     2161 gctattggtg atttcgtcat aggaacattt atcccactcg agagcaagaa gccaaaaggg
     2221 ttttttggct ataaatctga aatctttaat gagaactttg gccccgattt tcgagaagaa
     2281 gaaactttct tttctgtatt tgccatcttt tttcctgctg caactggaat tctggctggc
     2341 gcaaatatct caggtgatct tgcagatcct cagtcagcca tacccaaagg aacactccta
     2401 gccattttaa ttactacatt ggtttatgta ggaattgccg tgtctgtagg ttcttgtgtc
     2461 gtacgcgatg ccactggtaa tgttaatgac actatcgtaa cagagctaac aaactgtact
     2521 agcgcagcct gcaaattaaa ctttgattac ccttacgatg tccctgacta cgccggctac
     2581 ccatacgacg tgccagacta cgctttttca tcttgtgaat ccagtccttg tagctacggc
     2641 ctaatgaaca actttcaggt aatgagtatg gtgtcaggat ttacaccact aatttccgcg
     2701 ggcatattct ctgccactct ttctagcgca ttagcatccc tagtgagtgc tcccaagata
     2761 tttcaggccc tatgtaaaga caacatctac cccgctttcc agatgtttgc taaaggttat
     2821 gggaaaaata atgagccatt acgtggctac atcttaacat tcttaattgc acttggattc
     2881 atcttaattg ccgaactgaa tgttattgca ccaattatct caaacttctt ccttgccagc
     2941 tatgccctca tcaatttttc agtattccat gcttcactgg caaaaagccc aggatggcga
     3001 ccggcattta aatactacaa tatgtggatc tcacttcttg gagcaattct ttgttgcata
     3061 gtaatgttcg tcattaactg gtgggctgct ttgctaacct acgtgatagt ccttgggctg
     3121 tatatttatg ttacctacaa aaaacccgat gtgaattggg gatcctctac acaagccctg
     3181 acttacctca atgccctgca acatagtatt aggctttctg gcgtggagga ccatgtgaaa
     3241 aactttaggc cacagtgtct tgttatgaca ggagcgccaa actcacggcc ggccttactt
     3301 catcttgttc acgatttcac aaaaaatgtt ggtttgatga tctgtggaca cgtacacatg
     3361 ggaccacgca gacaagccat gaaagaaatg tccatcgacc aagccaaata tcagcgctgg
     3421 cttattaaga acaaaatgaa ggctttttat gcaccagtac atgcagatga cctcagagaa
     3481 ggggcccaat acttgatgca ggctgctggc ctgggccgta tgaagccaaa cacactagtc
     3541 cttggattta agaaagattg gttgcaagca gatatgaggg atgtggatat gtacataaac
     3601 ttatttcacg atgcttttga catacaatat ggcgtagtgg ttattcgcct aaaagaaggt
     3661 ctggatatta gtcacctgca gggacaagaa gaattattgt catcacaaga gaaatctcct
     3721 ggcaccaagg atgtcgtagt aagtgtggaa tatagtaaaa agtccgattt agatacttcg
     3781 aaacccctca gtgaaaaacc aattacccat aaagtcgagg aagaagatgg caagactgca
     3841 actcaaccac tgttgaaaaa agaatccaaa ggccctattg tgcctttgaa tgtagctgac
     3901 caaaagcttc ttgaagctag tacacagttt cagaaaaaac aaggaaagaa tactattgat
     3961 gtctggtggc tttttgatga cggaggtctg accttattga taccttacct tctgacgacc
     4021 aagaaaaaat ggaaagactg taagatcaga gtattcattg gtggaaagat aaacagaata
     4081 gaccatgacc ggagagcgat ggctacttta ctgagcaagt tcagaatcga tttttctgac
     4141 atcatggttc taggagacat caataccaaa ccaaagaaag aaaacattat agctttcgag
     4201 gaaatcattg agccatacag acttcacgaa gatgataaag agcaagatat cgcagataaa
     4261 atgaaagaag atgaaccctg gcgaataaca gataatgaat tggagctgta taagaccaag
     4321 acataccggc agatcaggtt aaatgagtta ttaaaggaac attcaagcac agctaacatt
     4381 attgtgatga gtttaccggt cgcacgaaag ggtgccgttt ctagtgctct ctacatggca
     4441 tggttagaag cactatctaa ggatctacca ccaatcctac tagttcgtgg gaatcatcag
     4501 agtgtcctta ccttctattc ataattaatt aagtttaaac ccgctgatca gcctcgactg
     4561 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     4621 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     4681 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4741 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4801 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4861 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4921 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4981 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5041 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5101 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5161 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5221 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5281 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5341 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5401 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5461 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5521 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     5581 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     5641 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     5701 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5761 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5821 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     5881 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5941 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6001 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6061 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6121 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6181 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6241 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6301 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6361 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6421 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     6481 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     6541 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6601 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     6661 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6721 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     6781 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6841 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6901 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7021 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7081 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7141 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7201 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7621 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7681 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7741 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7801 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7861 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7921 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7981 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8041 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8101 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8161 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8221 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8281 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8341 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8401 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8461 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8521 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8581 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8641 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8701 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8761 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8821 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8881 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8941 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     9001 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     9061 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     9121 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     9181 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     9241 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     9301 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     9361 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     9421 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     9481 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     9541 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     9601 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     9661 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     9721 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     9781 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     9841 agacccaagc tg
//
