LOCUS       sequence-72205-e        9984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian 
            cells. cDNA is synthetic, containing convenient restriction sites. 
            Native hNKCC1 amino acid sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Somasekharan S, Monette MY, Forbush B
  TITLE     Functional Expression of Human NKCC1 from a Synthetic Cassette-Based
            cDNA: Introduction of Extracellular Epitope Tags and Removal of 
            Cysteines.
  JOURNAL   PLoS One. 2013 Dec 5;8(12):e82060. doi: 
            10.1371/journal.pone.0082060.
  PUBMED    24339991
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-5"; volume: "8(12):e82060. doi"; pages: " 
            10.1371/journal.pone.0082060"
COMMENT     Sequence Label: pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)
FEATURES             Location/Qualifiers
     source          1..9984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..117
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDY"
     CDS             join(130..132,133..843)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(172..193)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(433..452)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     780..801
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4515..4532)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4521..4745
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4791..5219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4878..4897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5088..5109
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5228..5248)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5233..5562
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5413..5548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5475..5494
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5611..6636
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5611..6636
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6766..6887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6803..6822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6857..6876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6936..6952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6936..6952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6949..6971)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6960..6976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6984..6990,6991..7008,7009..7014))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7029..7050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7167..7184)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7338..7923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7418..7437)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8094..8885,8886..8954))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8717..8736
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8955..9059)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9134..9153)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9325..9704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9705..9908
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9859..9879
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9953..9972
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9953..9971
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctagcccgg gcacggccgc caccatggga tacccttacg atgtccctga ctacgccggc
       61 tacccatacg acgtgccaga ctacgctggg tcctacccct acgacgttcc cgattacggt
      121 gaccctaccg tcagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      181 ctggacggcg acgtaaacgg ccacaagttc tcggtctctg gcgagggcga gggcgatgcc
      241 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      301 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcgctcgcta ccccgaccac
      361 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      421 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      481 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      541 gggcacaagc tggagtacaa cgccatcagc gacaacgttt atatcaccgc cgacaagcag
      601 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      661 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      721 aaccactacc tgagcaccca gtccaaactg agcaaagacc ccaacgagaa gcgcgatcac
      781 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctctac
      841 aagggtgacc tggagccgag gcccacggct cctagctccg gcgcgccagg cctcgccgga
      901 gtcggcgaaa cgccgagcgc cgctgcgctg gccgcagcca gggtggaact gcccggaacc
      961 gctgtgccct cggtgccgga ggatgctgcg cccgcgagcc gggacggggg cggggtccgc
     1021 gatgagggac cagccgccgc cggtgacggc ctgggcagac ccttggggcc taccccgagc
     1081 cagagccgtt tccaggtgga cctcgtttcc gagaacgcag ggcgggctgc tgctgcggcg
     1141 gcggcggctg ctgcggccgc cgccgctgct ggtgccgggg cgggggccaa gcagactcct
     1201 gccgacgggg aagccagcgg cgagagcgag ccagctaaag gcagcgagga agccaaaggc
     1261 cgcttccgcg ttaacttcgt agatcctgct gccagctcgt ccgcagaaga tagcctgtca
     1321 gatgccgccg gggtcggagt cgatggaccc aacgtgagct tccagaacgg cggggacacg
     1381 gtactgagcg agggcagcag cctccattcc ggtggcggcg gcggctccgg ccaccaccag
     1441 cactactatt atgataccca caccaacacc tactatctgc gcaccttcgg ccacaacaca
     1501 atggacgcgg tacccaggat cgaccactac cggcacacag ccgcgcagct cggcgagaag
     1561 ctgctccggc ctagcctggc cgagctgcat gacgagctgg aaaaggaacc ttttgaggat
     1621 ggctttgcaa atggggaaga aagcactcca acacgtgatg ctgtggtcac gtacacggcc
     1681 gaaagtaaag gagtcgtgaa gtttggctgg atcaagggtg tactagtacg ttgtatgtta
     1741 aacatttggg gtgtgatgct tttcattcgt ctctcatgga ttgtgggaca agctggaatt
     1801 ggtctatcag tccttgtaat aatgatggct actgttgtga caactatcac aggattgtcc
     1861 actagcgcca tcgcaactaa tggatttgta aggggaggcg gcgcatatta tttaatatct
     1921 agaagtctag ggcctgaatt tggtggtgcc attggtctaa tcttcgcctt tgccaacgct
     1981 gttgctgttg ctatgtatgt ggttggattt gcagaaaccg tggtggagtt gctgaaagaa
     2041 cattccattc ttatgataga tgaaatcaat gacatccgga ttattggagc cattacagtc
     2101 gtgattcttt taggtatctc agtagccggt atggagtggg aagcaaaagc tcagattgtt
     2161 cttttggtga tcctacttct tgctattggt gatttcgtca taggaacatt tatcccactc
     2221 gagagcaaga agccaaaagg gttttttggc tataaatctg aaatctttaa tgagaacttt
     2281 ggccccgatt ttcgagaaga agaaactttc ttttctgtat ttgccatctt ttttcctgct
     2341 gcaactggaa ttctggctgg cgcaaatatc tcaggtgatc ttgcagatcc tcagtcagcc
     2401 atacccaaag gaacactcct agccatttta attactacat tggtttatgt aggaattgcc
     2461 gtgtctgtag gttcttgtgt cgtacgcgat gccactggta atgttaatga cactatcgta
     2521 acagagctaa caaactgtac tagcgcagcc tgcaaattaa actttgattt ttcatcttgt
     2581 gaatccagtc cttgtagcta cggcctaatg aacaactttc aggtaatgag tatggtgtca
     2641 ggatttacac cactaatttc cgcgggcata ttctctgcca ctctttctag cgcattagca
     2701 tccctagtga gtgctcccaa gatatttcag gccctatgta aagacaacat ctaccccgct
     2761 ttccagatgt ttgctaaagg ttatgggaaa aataatgagc cattacgtgg ctacatctta
     2821 acattcttaa ttgcacttgg attcatctta attgccgaac tgaatgttat tgcaccaatt
     2881 atctcaaact tcttccttgc cagctatgcc ctcatcaatt tttcagtatt ccatgcttca
     2941 ctggcaaaaa gcccaggatg gcgaccggca tttaaatact acaatatgtg gatctcactt
     3001 cttggagcaa ttctttgttg catagtaatg ttcgtcatta actggtgggc tgctttgcta
     3061 acctacgtga tagtccttgg gctgtatatt tatgttacct acaaaaaacc cgatgtgaat
     3121 tggggatcct ctacacaagc cctgacttac ctcaatgccc tgcaacatag tattaggctt
     3181 tctggcgtgg aggaccatgt gaaaaacttt aggccacagt gtcttgttat gacaggagcg
     3241 ccaaactcac ggccggcctt acttcatctt gttcacgatt tcacaaaaaa tgttggtttg
     3301 atgatctgtg gacacgtaca catgggacca cgcagacaag ccatgaaaga aatgtccatc
     3361 gaccaagcca aatatcagcg ctggcttatt aagaacaaaa tgaaggcttt ttatgcacca
     3421 gtacatgcag atgacctcag agaaggggcc caatacttga tgcaggctgc tggcctgggc
     3481 cgtatgaagc caaacacact agtccttgga tttaagaaag attggttgca agcagatatg
     3541 agggatgtgg atatgtacat aaacttattt cacgatgctt ttgacataca atatggcgta
     3601 gtggttattc gcctaaaaga aggtctggat attagtcacc tgcagggaca agaagaatta
     3661 ttgtcatcac aagagaaatc tcctggcacc aaggatgtcg tagtaagtgt ggaatatagt
     3721 aaaaagtccg atttagatac ttcgaaaccc ctcagtgaaa aaccaattac ccataaagtc
     3781 gaggaagaag atggcaagac tgcaactcaa ccactgttga aaaaagaatc caaaggccct
     3841 attgtgcctt tgaatgtagc tgaccaaaag cttcttgaag ctagtacaca gtttcagaaa
     3901 aaacaaggaa agaatactat tgatgtctgg tggctttttg atgacggagg tctgacctta
     3961 ttgatacctt accttctgac gaccaagaaa aaatggaaag actgtaagat cagagtattc
     4021 attggtggaa agataaacag aatagaccat gaccggagag cgatggctac tttactgagc
     4081 aagttcagaa tcgatttttc tgacatcatg gttctaggag acatcaatac caaaccaaag
     4141 aaagaaaaca ttatagcttt cgaggaaatc attgagccat acagacttca cgaagatgat
     4201 aaagagcaag atatcgcaga taaaatgaaa gaagatgaac cctggcgaat aacagataat
     4261 gaattggagc tgtataagac caagacatac cggcagatca ggttaaatga gttattaaag
     4321 gaacattcaa gcacagctaa cattattgtg atgagtttac cggtcgcacg aaagggtgcc
     4381 gtttctagtg ctctctacat ggcatggtta gaagcactat ctaaggatct accaccaatc
     4441 ctactagttc gtgggaatca tcagagtgtc cttaccttct attcataatt aattaagttt
     4501 aaaccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4561 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4621 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4681 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4741 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4801 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4861 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4921 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4981 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5041 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5101 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5161 ggccatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5221 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5281 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5341 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5401 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5461 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5521 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5581 atatccattt tcggatctga tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct
     5641 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     5701 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     5761 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     5821 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     5881 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     5941 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     6001 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     6061 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     6121 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     6181 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     6241 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     6301 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     6361 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     6421 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     6481 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     6541 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     6601 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tgctacgaga tttcgattcc
     6661 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6721 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6781 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6841 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6901 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6961 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7021 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7081 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7141 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7201 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7261 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7321 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7381 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7441 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7501 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7561 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7621 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7681 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7741 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7801 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7861 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8101 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8161 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8221 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8281 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8341 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8401 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8461 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8521 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8581 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8641 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8701 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8761 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8821 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8881 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8941 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9001 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9061 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     9121 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     9181 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     9241 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     9301 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     9361 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     9421 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     9481 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     9541 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     9601 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     9661 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     9721 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     9781 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     9841 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     9901 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
     9961 ctcactatag ggagacccaa gctg
//
