LOCUS       sequence-72206-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Hong TT, Smyth JW, Gao D, Chu KY, Vogan JM, Fong TS, Jensen BC, 
            Colecraft HM, Shaw RM
  TITLE     BIN1 localizes the L-type calcium channel to cardiac T-tubules.
  JOURNAL   PLoS Biol. 2010 Feb . 8(2):e1000312.
  PUBMED    20169111
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2010 Feb . 8(2):e1000312."
COMMENT     Sequence Label: pDest-eGFP-N1
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(77..82,83..100,101..107)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             161..841
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(227..246)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
       61 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
      121 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      181 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      241 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      301 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      361 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
      421 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
      481 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
      541 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
      601 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
      661 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
      721 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
      781 attacaacag tactgcgatg agtggcaggc ggggcgtaat ctagaggatc cggcttacta
      841 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtat
//
