LOCUS       sequence-72214-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length SPATR with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: SPATR-bio
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(816..835)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 cctcactctt ataggccgtg atggaggtcg acggcgcgcc gcagcttctg gtctgttcca
       61 tgatgttgcg gggcacgatc ttgtcgttcc acaggcagaa gttctcattg ttctggttgc
      121 tccgccgcac ttttctgtac cgtttctggg cattgttctt gcaggcgctc caatcgctcc
      181 aaggggtgta ggcgcagttc acgttgtcgt tctccacctt gcggcacagg tagaagttgt
      241 tcacggtgca cttccggggc acgtaccaag ggttccgctt ctgttcttcg atcatttcct
      301 cgccagtgat atagccgctg gcgccgatga tctcgaagtc ggcgtagtcc ttgttgctgc
      361 cgtccttaca cagcacagcg agttcgtagt cgatgaccac gctcatgctc ttgaagccca
      421 cgtcgttgct gttgtcgcac agccgcttca tccacagggg ggtgaccagg ctccgctcgt
      481 gcttgttctt gatctcgttc agcttgccct gcacgtccag cacgtcctct tcgctgcaca
      541 ggatatagcc tctagggcac cagcagtttc tgctgttgcc ctcgctgctg ctgaagatca
      601 cacaggtgtc gccgtccacg tcgggctggc tctccacgtc gcccttgatc ttttcctccc
      661 ggatgttgtc gttgttcttg ttggtctgca ggctgttgat ctccaccttc tgcttccgct
      721 tgaactcggc ggccgcacca gacatgcaga gcagcaggga catgagtacc tgggtctgaa
      781 actccatggt ggcaaacttg gacctgggag tggacacctg tggagagaaa ggcaaagtgg
      841 atgtcattgt cactcaagtg tatggccaga tcgggccagg tgaatatcaa atcctcctcg
      901 tttttggaa
//
