LOCUS       sequence-72216-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV TALE synthesis backbone with NN half repeat; fused to nuclear 
            localization sequence linker and SID4X transcriptional repressor 
            effector domain. Synapsin promoter for neuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: 
            pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..74)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(767..793)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cgttccaggt agtcagcggc ctccagcagc atctggatgt tcatgctggc ctccaccttt
       61 ctcttcttct tggggctgct agctgccact cgatgtgatg tcctctcggg aatccttctg
      121 ttggtccgct taatcaatgc aggcgcgtgc gggagcccct tcttcaccgc atccaaggcg
      181 ggtcgtccgc cgaggcaggc cagcgctaca agatgatcat tagtgagtgc ggccagcgcg
      241 gggtccggcc tcgaaagctg ggccacgatt gactccagtg cgggtctgcc cccgttattg
      301 gatgcaatag ccactacctg ctcaggcgtg agtcgagacc cgtacgggtc tctgtcaggt
      361 tgaggggtgc tcccgtgagc gcattgcgcc acgcgtgcac cgcctcgacc gccgtgactc
      421 ctccccgctt cgcgatcttc agcaactggc ccgtgtccag ctgaagggga ggccctctca
      481 gctctcccgc gaccgtcaac agggcctcaa gcgctcgggc tccgctccac tgtttcccca
      541 caccgacgat cgcctcatgt gtggcttccg gcaacgccgc aatcatgtcc tggtacttga
      601 cggcgaccgt gccaagggct gcagggtgct gcgacaaggc tacgatgtgg gcatgtgtaa
      661 acccatgacc caccagcgct tcgtgatgct gcgcgactgt cgacctcact ttgggcttga
      721 tcttttcctg ctgctgctgt gaatatccca aagttctcaa atctacagcg taatctggaa
      781 catcgtatgg gtacatggtg gcggatcctt ctcgactgcg ctctcaggca cgacacgact
      841 cctccgctgc ccaccgcaga ctgaggcagc gctgagtcgc cggcgccgca gcgcagatgg
      901 tcgcgcccgt gcccccctat ctcgcgcctc gcgtggtgcg gtccggctgg gccggcggcg
      961 gcgcggacgc gaccaaggtg gccgggaagg ggagtttgcg g
//
