LOCUS       sequence-72217-e        1121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV TALE synthesis backbone with NN half repeat; fused to nuclear 
            localization sequence linker and SID4X transcriptional repressor 
            effector domain. Synapsin promoter for neuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: 
            pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
FEATURES             Location/Qualifiers
     source          1..1121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(396..449)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(849..869)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tcttacacat gatcacttcc caccagctgc acaccgatga agtattgcag ccctcccttt
      121 cgatcccgga caggttggag atgaagcaga ttccagaact ttttgcctga cttagtgtag
      181 tttattaact gcacagtggt ttccctctga tcgcggatag catcgcgaat cttctgaacg
      241 gtggcctggt ctgtctcagg cccttgcagg aacctggcgt ttctccccat gatttcctcc
      301 ctggaatatt cggtgagctc cagaaaaccg tcgctggcaa aaatgatggg gtaatcaggc
      361 agtcttggat ctgttattac gaaggacttc tcgattgggc caggattctc ctcgacgtca
      421 ccgcatgtta gcagacttcc tctgccctct ccactgcccc atgggggcag catagaggca
      481 tagccatgtt cagcttctct ctcgcgcctc tccaggtaat cggcagcctc cagcagcatt
      541 tgtatgttca tcccgctgcc tggcagcatg ctggcgtatc cgtgctcggc ttctctttct
      601 ctccgttcca ggtaatcggc tgcctccagc agcatttgga tattcattcc agacccaggc
      661 agcatggaag catagccatg ttcggcctcc cgttcgcgcc tttccaggta atcagcagcc
      721 tccagcagca tctgaatatt catgccgctt ccgggcagca ttgaagcata tccgtgctcg
      781 gcttcgcgct ctctccgttc caggtagtca gcggcctcca gcagcatctg gatgttcatg
      841 ctggcctcca cctttctctt cttcttgggg ctgctagctg ccactcgatg tgatgtcctc
      901 tcgggaatcc ttctgttggt ccgcttaatc aatgcaggcg cgtgcgggag ccccttcttc
      961 accgcatcca aggcgggtcg tccgccgagg caggccagcg ctacaagatg atcattagtg
     1021 agtgcggcca gcgcggggtc cggcctcgaa agctgggcca cgattgactc cagtgcgggt
     1081 ctgcccccgt tattggatgc aatagccact acctgctcag g
//
