LOCUS       sequence-72227-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     Jorgensen unpublished mosSCI reagents
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ210
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(88..94,95..112,113..118))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(143..266)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(274..291)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(274..290)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
       61 tcgacggatc ctaactcaaa atccacacat tatacgagcc ggaagcataa agtgtaaagc
      121 ctggggtgcc taatgcggcc gccatagtga ctggatatgt tgtgttttac agtattatgt
      181 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      241 tcgttcaact ttattataca tagttgataa ttcactggcc gtcgttttac actcgagacg
      301 tacggtgcgc gcgatgcatt cgaagatctg cccactagtg agtcgtatta taagtgcaag
      361 taagatcagt gtttgtattt cagtaatcaa ttatttttcg ctcccccctc ctcgcaagtc
      421 ggcgatttct ttgaagtttt gtcaaaagaa gagacaagga cttgatagat acgctcatca
      481 gagtgagatt cattgcaaat agtgtgtaaa tcgtggagag acgagagaaa aggtgtgaga
      541 agcttagaag ataaagaata ggaagaagaa catatctcat ttcctatttt ctctaatttt
      601 tcgttcattt ttctagacat tacccatttg atggtttttc aatatggtta tcagggtact
      661 cattttggta gttttcaaag caatttggag ttttttttta aattgtcaac tacaaaaacg
      721 cagcttaaag gtccaaatat catagtataa aaattcttta aaaaattcca aattttatat
      781 gattttttga aaattggaag
//
