LOCUS       sequence-72234-e        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal 
            alpha-importin nuclear localization sequence. EF1-alpha promoter for
            ubiquitous mammalian expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2013 Jul 23. doi: 10.1038/nature12466."
COMMENT     Sequence Label: LITE2.0
            EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2...
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..83)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(105..254)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(279..299)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcact gggccaggat tctcctcgac gtcaccgcat
       61 gttagcagac ttcctctgcc ctctccactg cctctagagt taatcagcat gtccaggtcg
      121 aaatcatcaa gggcgtcact gccgagcatg tcgaggtcaa agtcatcaag ggcatccgaa
      181 ccaagcatgt caaggtcaaa atcatcgagg gcgtcacttc ccagcatatc cagatcaaaa
      241 tcgtccaatg cgtcagcccg tccggaaccg ctggcctcca cctttctctt cttcttgggg
      301 ctagccggcg ctgctccgat cataatctgc gcttcgcgtg ttcttgagat ggctttcgcc
      361 agcaattctc gtgcggtgtc aatatcgacg atgggtttcg cgtaattcgt ccccaattcg
      421 acccctgagg ctttaaggac ggtcagggga gcatcccacg ggtggtgaat ccactcggtg
      481 ggcagacggg ccagctctgg gagccactgc ctgatgtatt ctccctccgg gtcgtacttt
      541 gctccttgca gtgcagggtt atccaatcgg tcaagttcat gcccgtcagg gatggaaccc
      601 gaaatgtact gccacccaag aatatcgcac tccaagtccg catccagaag cgtgtcccaa
      661 aagtatttca tcccccattt ccagggcaac aagaggaact taacggcaaa gctactaacg
      721 atgactcgaa tacggttgtg catccaaccc gttgcccaca gctctctcat acccgcatcc
      781 accaacgggt atcccgtgcg cccctgccgc caggccttga atttgtccac gtccgcgtcc
      841 cacggaaaga agcggaggtg gctgaggagg gattgttcat gtgtaaacgg aaagttgaag
      901 cagatgtatc gtgaatactc cctaaggccg attcctctca aaaacaaatc ggcagactcc
      961 tctccttcag agtttttgtc gcgagcccaa atgatttgct tcatccgcgc acattggaac
     1021 acatgcctca cgctaatctc cccaaagtgg agatacggcg
//
