LOCUS       sequence-72260-e         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid with mRuby2 for Golden Gate reaction of TALE 
            assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
  TITLE     Live visualization of chromatin dynamics with fluorescent TALEs.
  JOURNAL   Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680.
  PUBMED    24096363
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680."
COMMENT     Sequence Label: pTALYM4
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    320..341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(356..361,362..379,380..386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    394..410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     399..421
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     418..434
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     418..434
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     453..473
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        455..473
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(520..539)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(521..539)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(549..566)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(549..565)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(558..580)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcacctgtgc atttgaattg gtggccgttg accgaacctt ccatgaccac cttcatacgc
       61 atattttcct tgatcagctc ttcgccctta gacaccatgg aggaggcggt gccggagcca
      121 cctccgcctc cagtggatcc cgcaacgcga tgggacgtgc gttcgccaat acggcgattg
      181 actcttctga tcaattccgg cgcgtgcggc aatccctttt tcactgcatc catggcagga
      241 cgtccgccga ggcaggccaa ggcgacgagg tggtcgttgg agacgaccgg tggaaagcgg
      301 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      361 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
      421 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
      481 agctggagga cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac
      541 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      601 cttaatcgcc ttgcagcaca tccccctttc gc
//
