LOCUS       sequence-72263-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant protein production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, 
            Sergeev YV
  TITLE     Functional validation of hydrophobic adaptation to physiological 
            temperature in the small heat shock protein alphaA-crystallin.
  JOURNAL   PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 
            2012 Mar 29.
  PUBMED    22479631
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e34438. doi"; pages: " 
            10.1371/journal.pone.0034438. Epub 2012 Mar 29"
COMMENT     Sequence Label: Danio rerio cryaa
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             585..602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(655..673)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      669..716
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agaaggagat atacatatgg atattgcgat ccaacaccct tggttcagac gcacactggg
       61 ctaccccacc cgactctttg atcagttctt tggagaaggc ctgttcgatt atgacctatt
      121 ccccttcacc acttcaactg tcagccctta ctatcgacac tcactcttcc gcaacatcct
      181 ggactcctcc aactcaggtg tctctgaggt gaggtctgac agagaaaaat ttacagttta
      241 tctggatgtg aaacatttct ctcctgatga gctcagtgtc aaggtgacag atgactatgt
      301 ggagatccag ggcaagcatg gagaaagaca ggatgatcat ggctacatct cccgtgagtt
      361 ccatcgtcgc taccgcctgc cttccaatgt ggaccagtct gccatcacct gcacactgtc
      421 tgctgatggc ctgctcactc tttgtggacc caagacaagt ggcatagatg ctggccgtgg
      481 agatcgcacc atccctgtta cccgcgagga caagagcaac tcaggctctt cctcctaggg
      541 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
      601 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      661 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      721 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      781 ggcgggtgtg gtggttac
//
