LOCUS       sequence-72264-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination plasmid with mRuby2 for Golden Gate reaction of TALE 
            assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Miyanari Y, Ziegler-Birling C, Torres-Padilla ME
  TITLE     Live visualization of chromatin dynamics with fluorescent TALEs.
  JOURNAL   Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680.
  PUBMED    24096363
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680."
COMMENT     Sequence Label: pTALYM4
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(5..24)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6..24)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(34..51)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(34..50)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(43..65)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(626..646)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
       61 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      121 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      181 tggcgaatgg gacgcgccct gtagcggcgc ccgtctccag gggggcaccc gtcagtgcat
      241 tgcgcgatgc atgcactgcc tccattgcgg tcacgccgcc acgttttgca atcttcacaa
      301 gttggcctgt gtccaactgt aacggcggac ctctcaactc ccccgcatcc gtgagcaagg
      361 cctccagggc gcgtgcgccg gaccactgtt tgccgacgcc aacgatgtct tcgtgtgtcg
      421 cctctggcaa cgccgtgatt atgtgctgat acgtgacagc gacggtccct aacgctgccg
      481 ggtgttggct gagcgcaacg atgtgcgcgt gtgtaaaccc atggcccacc agtgcctcnt
      541 ggtgctgcgc cactgtcgaa cgcaccttcg gtttgatctt ctcttgctgc tgctgactgt
      601 agccgagcgt gcgtagatcc acctgcacct tcctcttttt cttaggagat ctcatggtgg
      661 cggcccctat ag
//
